In vitro fertilization (IVF) can facilitate the recovery and expansion of mouse lines as sperm from one mouse can potentially produce hundreds of offspring using IVF. IVF is used to recover frozen sperm and be used to expand new strains brought in from other universities. This technique also has the potential to rescue valuable strains that fail to breed.
Investigators must supply the following items
- Completed Service Request form
- At least 4 males, 8 weeks to 4 months old, or frozen sperm sample
- If homozygous offspring are essential, at least 16 females, 3-4 weeks old, are required
- Current protocol number for the pups
- IT form for payment (current charge is $1200, Tulane investigators only)
- Mice arrive
- IVF set up, eggs transferred to foster mother
- Foster mice give birth (investigator notified)
- Tail tips given to investigator when pups are 15-16 days old
- Pups are weaned at 21 days old
- The entire process normally takes 7-8 weeks
If the investigator wishes to rederive mice via IVF, additional charges will apply.
IVF with live birth and cryopreservation of embryos and can be combined for an additional charge. In addition, more females are required for this procedure.
If the foster females fail to deliver live pups, the process will be repeated at no additional cost (other than the cost of more mice, if needed).
Please send an e-mail to Ann Mullin for pricing information. Outside investigators must be at an institution based in Louisiana.
The arrival of mice from any non-approved vendor must be coordinated with the director of the Uptown Vivarium, Amy Pierce.
The Tulane Transgenic Mouse Facility wishes to acknowledge the Tulane Research Enhancement Fund for awarding funds for the purchase of equipment for IVF and cryopreservation.